Halorubrum salsamenti sp. nov., a Novel Halophilic Archaeon Isolated from a Brine of Salt Mine

Abstract

A non-motile, spherical or oval extremely halophilic archaeon, strain Y69^T, was isolated from a brine of the Yunnan salt mine, China. Colonies on JCM 168 agar plate were round (1–2 mm in diameter), moist, and orange-pigmented. Phylogenetic analysis of the almost-complete 16S rRNA gene sequence showed that the isolate belonged to the species of the genus Halorubrum, with a close relationship to Halorubrum aidingense 31-hong^T (98.5%), Halorubrum lacusprofundi ATCC 49239^T (98.2%), and Halorubrum kocurii BG-1^T (98.0%). The major polar lipids of strain Y69^T were phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. Strain Y69^T grew in 15–30% (w/v) NaCl. The temperature and pH ranges for growth were 25–50 °C and 6.5–9.0, respectively. Optimal growth occurred at 20% (w/v) NaCl, 42 °C, and pH 8.0. Mg²⁺ was required for growth. The DNA G+C content was determined to be 65.1 mol% by the thermal denaturation method. DNA–DNA hybridization values between strain Y69^T and the closely related species were lower than 70%. Based on the data presented in this study, strain Y69^T represents a novel species for which the name Halorubrum salsamenti sp. nov. is proposed. The type of the strain is Y69^T (=CGMCC 1.15455^T = JCM 31270^T).

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